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KMID : 0603520010060030155
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Journal of Korean Association of Cancer Prevention
2001 Volume.6 No. 3 p.155 ~ p.164
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Ofloxacin Inhibits Hypoxia/Hypoglycemia-Induced Apoptosis in Bovine Aortic Endothelial Cells
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Kim Hae-Sun
Lee You-Mie
Yoo Mi-Ae
Lee Jong-Won
Ryu Seuk-Hwan
Kim Kyu-Won
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Abstract
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Hypoxia/hypoglycemia can cause apoptosis, an important feature in the progression of ischemic diseases such as ischemic infarction, stroke and athroscleosis. It was reported that previous use of the flouquinolone type antibiotic ofloxacin decreases the risk of developing acute myocardial infarction. On the basis of previous reports, we hypothesize that ofloxacin may prolong survival of endothelial cells against hypoxia/hypoglycemia-induced apoptosis. The ofloxacin treatment increased the viability of BAECs (Bovine Aortic Endothelial Cells) exposured to hypoxia/hypoglycemia for 48h compared with the control. Ofloxacin reduced the DNA fragmentation and the nuclear condensation. By using FACSscan analysis after PI staining, we found that the proportion of apoptotic subdiploid cells was decreased by the treatment of ofloxacin under hypoxia/hypoglycemia. In order to investigate the anti-apoptotic mechanism of ofloxacin under hypoxia/hypoglycemia, we examined the expression levels of antiapoptotic protein Bc1-2 and proapoptotic protein Bax by immunoblot analysis. The treatment of ofloxacin induced the relatively higher expression levels of Bc1-2 in BAECs under hypoxia/hypoglycemia. However, the expression levels of Bax were not changed. Taken together, these results suggest that ofloxacin inhibits hypoxia/hypoglycemia-induced apoptosis and increases the viability of BAECs through enhancement of Bc1-2 protein expression under hypoxia/hypoglycemia.
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KEYWORD
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Ofloxacin, Hypoxia, Hypoglycemia, Apoptosis
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